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Department of Biotechnology Anna University

Jaisy Samuel


Teaching Fellow, Centre for Biotechnology

Department of Biotechnology

Anna University, Chennai 600025


044 - 2235 8395

jaisysamuel@yahoo.co.in







Academic Qualifications

School of Medicine, Deakin University, Australia from June 2010 to February 2015; Doctor of Philosophy; Thesis title: Targeting the Sodium Iodide Symporter (NIS) and apoptotic genes in extrathyroidal tumors.
Vellore Institute of Technology University (VIT), Vellore, India from August 2007 to August 2009; M.Sc. Biotechnology with CGPA of 8.82/10.
Vellore Institute of Technology University (VIT), Vellore, India from June 2004 to May 2007; B.Sc. Biotechnology with CGPA of 9.10/10.


Experience

October 2016 to April 2017 Guest Lecturer, Madras University, Department of Biotechnology, Guindy campus, Chennai Courses Taught-Post Graduate: Nanobiotechnology, Molecular Cell Biology and Animal Cell Biotechnology
July 2018 to present Teaching Fellow, Anna University, Department of Biotechnology, Guindy campus, Chennai Courses Taught- Undergraduate and Post Graduate: Cell Biology, Animal Biotechnology, Basic Industrial Biotechnology, Cancer Biology, Biopharmaceutical Technology and Food Safety.


Scholarships, Fellowships, Distinctions Received

• VIT University Merit Scholarship in the year 2006.

• Student exchange program February 2010-July 2010inINSTITUT FÜR BOTANIK, LEIBNIZ UNIVERSITÄT, GERMANY.

• Project fellowship in a BARC sponsored project (BRN/35/17/2009) June 2010-December 2013.

• Recipient of travel grant award of 1200$ and waiver of 400$ registration fee in the Meeting of the International Society for Genetic Eye Diseases & Retinoblastoma (ISGEDR) Ghent, Belgium, August 22 – 24, 2013.






Past Research Experience


• Under the supervision of Dr. Mahaboob Khan Rasool, I had evaluated the hepatoprotective and antioxidant effects of Spirulina fusiformis (a cyanobacterium family Oscillatoriaceae) against acetaminophen induced hepatotoxicity in mice. This study demonstrated that acetaminophen increases serum TNF-α and Spirulina fusiformis treatment reduced significantly reduced the elevated TNF-α level in acetaminophen challenged mice.

• I had also evaluated the hepatoprotective effect of gallic acid (3,4,5-trihydroxybenzoic acid), a plant phenol in acetaminophen induced hepatotoxic mice and our findings reveal that gallic acidtreatment alleviates the progression of the inflammation mediator TNF-a in paracetamol-exposed mice.

• Under the supervision of Dr.JuttaPapenbrock, I had studied the homologous and heterologous expression of arabidopsissulphotransferase and D-Cysteine desulphhydrase proteins.

• Under the supervision of Prof.JagatKanwar and Dr. Rupinder Kanwar, I had studied the effects of bovine lactoferrin and survivin dominant negative mutant on Retinoblastoma cells.



A Brief Summary Of The Research Project And Contribution In Vision Research Foundation, Sankara Nethralaya


At Vision Research Foundation, SankaraNethralaya, Chennai, I was a key researcher in the area of “Retinoblastoma & Therapy” at the laboratory for Ocular Pathology. In the field of retinoblastoma my major focus was targeted radioiodine therapy to cancer cells to improve the impact of radionuclide therapy on cancer cells alone. My research has direct relevance in therapy of cancers.



IN VITRO CELL CULTURE AND BIOCHEMICAL ANALYSES


• Mammalian cell line (Retinoblastoma-Y79 and Weri, Breast cancer cell line-MCF 7 and MDA MB 453, Müller glial cell line-MIOM1, Cervical cancer cell line- HeLa, Bacterial culture (E.coli,), In vitro cytotoxicity test ( MTT), generated stable cell lines.

• Molecular biology techniques such as ELISA, qRT-PCR, Electrophoresis, Fluorescent Activated Cell Sorter (FACS), Isolation of plasmid and genomic DNA, Cloning of genes, Western blot

• Performed animal experiments.



MATERIALS AND CHARACTERIZATION


Fluorescent & Phase Contrast Microscopy and Dynamic light scattering (DLS).



TRAINING


• On-Job training in Animal Biotechnology, Madras Veterinary College. It included training in hybridoma technology, immunoelectrophoresis, Invitro fertilization, PCR, vaccine preparation and haemmagglutination assay.

• Training in Symed Biotechnologies, R&D, Chennai- Screening of glycohydrolase enzyme activities pertaining to tapioca cultivar soil and tuber tissue symbionts.






Publications


  • EvanPrince Sabina, Jaisy Samuel, SeguRajappaRamya, Smita Patel, NiharikaMandal, PreetyPranatharthiiharan, PunyaPallabi Mishra, MahaboobKhanRasool(2009) Hepatoprotective and antioxidant potential of Spirulina fusiformis on acetaminophen- induced hepatotoxicity in mice. International Journal of Integrative Biology. 2009 Feb(6):1-5.
  • MahaboobKhanRasool, EvanPrince Sabina, SeguRajappaRamya, PreetyPranatharthiiharan, Smita Patel, NiharikaMandal, , PunyaPallabi Mishra,Jaisy Samuel; Hepatoprotective and antioxidant effects of gallic acid in paracetamol- induced liver damage in mice; Journal of Pharmacy and Pharmacology; 2010 May;62(5):638-43.
  • Upregulation of sodium iodide symporter (NIS) protein expression by an innate immunity component: Promising potential for targeting radiosensitive retinoblastoma; Jaisy Samuel, Narinderbir Singh, JagatRakeshKanwar, SubramaniumKrishnakumar, RupinderKaur Kanwar; Experimental Eye Research; 2015; 139; 108-114.
  • A Study of Gene Expression of Survivin, its Antiapoptotic Variants, and Targeting Survivin In Vitro for Therapy in Retinoblastoma. Samuel, Jaisy MSc; Kanwar, Rupinder K. PhD; Kanwar, Jagat R. PhD; Khetan, Vikas MD; Krishnakumar, Subramanian MD; Journal of Pediatric Hematology/Oncology.2016 Oct;38(7):e230-42


Papers under review


  • Sodium Iodide Symporter (NIS) protein expression in Retinoblastoma: A clinicopathological report. J Samuel, R K Kanwar, J R Kanwar, V Khetan, P Rishi, S Krishnakumar.
  • Targeted therapeutic gene delivery by EpCAM aptamer using polyethyleneimine in a nanocomplex formulation. Jaisy Samuel, Rama Shankar Verma , Sailaja Elchuri , Jagat R Kanwar, Rupinder K Kanwar , Subramanian Krishnakumar.
  • A study of EpCAM specific Sodium Iodide Symporter (NIS) expression in breast cancer cell line. Jaisy Samuel, Rama Shankar Verma, Dharmu Indra , Jagat R Kanwar, Rupinder K Kanwar , Subramanian Krishnakumar.


Posters (International)


  • Second prize in poster presentation for “SODIUM IODIDE SYMPORTER IN RETINOBLASTOMA” in DIRI Symposium, Frontiers in Science-21-23 Nov.-2011, Deakin University, Australia, in association with TERI, Delhi.
  • Poster presentation on the topic “TARGETED GENE DELIVERY USING PEI IN EpCAM POSITIVE CELLS BY EpCAM APTAMER” in DIRI Symposium, Frontiers in Science-21-23 Nov.-2011, Deakin University, Australia, in association with TERI, Delhi.


Paper (International)


  • Paper presentation onSODIUM IODIDE SYMPORTER IS DIFFERENTIALLY EXPRESSED IN RETINOBLASTOMA AND CORRELATES WITH THE AGGRESSIVENESS OF TUMORSin Meeting of the International Society for Genetic Eye Diseases & Retinoblastoma (ISGEDR) Ghent, Belgium, August 22 – 24, 2013.


Other Relevant Information


  • Actively participated and harmonized the mammalian cell culture laboratories in the Department of Nano biotechnology at Vision Research Foundation, SankaraNethralaya, Chennai, India.
  • Handled the responsibilities as a Senior Research Fellow in a BARC/BRNS sponsored project (BRNS/35/17/2009) of grant Rs.28 lakhs.
  • In collaboration with Board of Research in Nuclear Sciences, Mumbai, I had worked with radioactive substances under the supervision of Dr.Archana Mukherjee.
  • I also had opportunity to work with Prof. Juttapapenbrock and group at INSTITUT FÜR BOTANIK, LEIBNIZ UNIVERSITÄT, GERMANY.


REFERENCES


  • Prof.Jagat Kanwar, Professor in nanomedicine, School of Medicine, Deakin University, Geelong, Australia, jagat.kanwar@deakin.edu.au
  • Dr.Rupinder Kanwar, Senior Research Fellow in nanomedicine, School of Medicine, Deakin University, Geelong, Australia, rupinder.kanwar@deakin.edu.au
  • Dr.Sailaja Elchuri, Associate Professor, Department of Nanobiotechnology, Vision Research Foundation, Sankara Nethralaya, Chennai, India. sailaja.elchuri@gmail.com
  • Dr.S.Krishnakumar, Deputy Director-Vision Research Foundation, Sankara Nethralaya, Chennai, India. drkk@snmail.org






Contact


Teaching Fellow, Centre for Biotechnology

Department of Biotechnology

Anna University, Chennai 600025

jaisysamuel@yahoo.co.in

044 - 2235 8395