TAMILSELVAN JAYAVELU

Education

Degree	Year	University	Major Subject Studied
Ph D	2003-2008	Department of Medical Biochemistry, University of Madras	Biochemistry
M.Sc	2000-2002	Department of Medical Biochemistry, University of Madras	Biochemistry
B.Sc	1997-2000	University of Madras	Biochemistry

Professional experience

Associate Professor (Centre for Biotechnology, Anna University, Chennai-600025, India) (Period 2010 – till date)
• Research: Understanding the evolution of organ specific metabolism and signaling as well as their status under pathology with special reference to nutrient sensing/homeostasis and organelle turnover.
• Teaching: Cell Biology, Molecular Biology and Genetic Engineering theory and lab courses for undergraduate and post-graduate students.

Postdoctoral Fellow (Period 2009-2010 – Department of Pharmaceutical & Biomedical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC, USA)
• Elucidate the downstream signaling pathways of mitochondrial biogenesis induced by the serotonin receptor agonists on primary cultures of renal proximal tubular cells.

Postdoctoral Fellow (Period 2008-2009 – Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, USA)
• Understanding mitochondrial respiratory stress signaling in cancer with a new perspective about the distinctive nature of NFкB (cRel:p50) activation in transgenic mice and c2c12 skeletal myoblast cell culture model.

Doctoral Graduate (Period: 2003-2008 – Department of Medical Biochemistry, University of Madras, Tamilnadu, India)
• Thesis title: Effects of L-Carnitine and DL-α-Lipoic acid on mitochondrial Bioenergetics with Special Reference to Cytochrome c Oxidase and Apoptosis in Skeletal Muscle of Aged Rats.
The objective of our research was to understand the mechanism(s) that are responsible for mitochondrial respiratory dysfunction and loss of skeletal muscle fibers during aging.

Hands-on skills

Laboratory animals handled: Wistar albino rats, C57BL/6J and Nude mouse models.

Establishment and maintenance of cell culture: Primary cultures of adipocytes (rat), astrocytes (rat) and renal proximal tubular cells (rabbit). Cell lines include C2C12, L6, H9C2, 3T3-L1, MIO- M1, etc.

Techniques: Western, Northern and Southern blots, Immunoprecipitation, Immunofluorescence, Flow cytometry, Real time- PCR, EMSA, Cloning, HPLC, Enzyme assays and kinetics, Cellular fractionation and purity assessment (Ultracentrifugation), Mitochondrial techniques – respiration (using Seahorse XFe24/96 instrument), swelling, potential. Basic bioinformatics.

Lectures delivered

• Delivered a Lecture on "Organelle turnover signaling" in Evolutionary Trends in Biochemistry and Molecular Biology Biotrendz 2012 organized by Department of Biochemistry, Bharathidasan University, Trichy, India (20-Sep-2012).

• Delivered a Lecture on "Unaided autophagy is not sufficient to determine mitochondrial quality control in adipose tissue of diet-induced diabetic mice" in 38th Mahabaleshwar seminar on Mitochondria, Metabolism and Energetics organized by Tata Institute for Fundamental Research (TIFR), Mumbai, Mahabaleshwar, India (27-Jan-2014).

• Delivered a Lecture on "Effects of insulin signaling on mitochondrial turnover by targeting autophagy and mitochondrial biogenesis" in 5th International Conference on Molecular Signaling organized by AU-KBC Research Centre, MIT Campus, Anna University (12-Jan-2017).

• Delivered a Lecture on “Biotin deprivation elicits endoplasmic reticulum stress by inhibiting autophagy in aging brain” at Department of Biotechnology, MEPCO Schlenk Engineering College, Sivakasi, India (21-Sep-2019).

• Delivered a lecture on “Biotin deprivation inhibits autophagy to elicit ER stress in the aging brain” at Department of Genetics, University of Madras (24-Feb-2020).

Visits abroad

• Visited Denmark Technical University, Denmark from 11-Jun-2010 to 06-Jul-2010. Purpose of visit: International Research Staff Exchange Programme (IRSES) funded by East West Food-European Union to develop collaboration in the area of metabolic engineering.

• Visited Wageningen University, The Netherlands from 10-Feb-2013 to 31-Mar-2013. Purpose of visit: International Research Staff Exchange Programme (IRSES) funded by East West Food-European Union to develop collaboration in the area of metabolic engineering.

S.No	Project details	Funding Agency	Amount (in INR)	Duration
1	Astroglial specific biotin deprivation in aging brain influences mitophagy and mitochondrial biogenesis	SERB	50,84,400	3 years (2021-24)

S.No	Project details	Agency	Status
1	Autophagy influences hyperglycemia/ hyperinsulinemia induced adipocyte dysfunction: A possible mechanism of insulin resistance	DBT	32,14,000	3 years (2011-14)
2.	Dysregulation of autophagy influences mitochondria function and biogenesis in adipocytes: A Fundamental approach to insulin resistance.	SERB	54,31,000	3 years (2012-15)
3.	Hyperinsulinemia influences autophagy and mitochondrial biogenesis: A possible mechanism of neuronal ageing	ICMR	23,48,000	3 years (2013-16)
4.	Hyperinsulinemia influences autophagy and endoplasmic reticulum turnover in ageing	UGC	14,29,000	3 years (2012-15)
5.	Differential anaplerotic flux of amino acids influences insulin sensitivity: Role of biotin on biosynthetic capacity of the cell	DST-SERB	28,85,000	3 years (2017-20)

S.No	Project details	Agency	Status
1.	Metabolic Engineering of L. lactis for the production of propionic acid (Role: Co-Investigator)	DBT	49,80,000	3 years (2013-16)
1.	Production of L-2-Aminobutyric acid from citraconate by biotransformation and cell-free system	BIRAC	36,55,000	1.5 years (2018-19)

Research Guidance

• Number of Ph.D Scholars Guided 3

• Number of Ph.D Scholars Guiding 1

• Number of M.S (By Research) Scholars Guided : 1 (Co-guide)

• Number of M.Tech. Projects Guided 22

• Number of M.Tech. Projects Guiding 2

Details of Ph.D/M.S thesis

• Distinct biotin utilization between adipose and brain and its effects on mitochondrial dynamics under aging (Doctoral thesis - 2020)

• Astroglial biotin deprivation inhibits autophagy to elicit endoplasmic reticulum stress by uncoupling branched chain amino acids-mTORC1 role in lipogenesis in the aging brain (Doctoral thesis - 2020)

• Biotin inhibits autophagy to uncouple adipocyte protein synthesis via differntial regulation of mTORC1 and endoplasmic reticulum stress signaling (Doctoral thesis - 2019)

• Carbon and graphene scaffolds for cancer cell growth (M.S. by research thesis - 2019)Teaching subjects

• Cell and Molecular biology • Genetic Engineering

Research Publications

https://orcid.org/0000-0002-7143-352X

Lodha D, Rajasekaran S, Jayavelu T, Subramaniam JR (2020). “Detrimental effects of fructose on mitochondria in mouse motor neurons and on C. elegans healthspan", NutrNeuroscidoi: 10.1080/1028415X.2020.1853413 (IF 2019: 4.028)

RamaianSanthaseela A, Jayavelu T*(2020). "Does mTORC1 inhibit autophagy at dual stages?". BioEssaysdoi: 10.1002/bies.202000187 (IF 2019: 4.080)

Ramaian Santhaseela A, Ganesan D, Rajasekaran S, Selvam S, Jayavelu T* (2020) “Astrocytes resolve endoplasmic reticulum stress through mitochondrial fusion facilitated by biotin availability” Cell stress and Chaperones doi: 10.1007/s12192-020-01129-6

Ramaian Santhaseela A, Ganesan D, Rajasekaran S, Selvam S, Jayavelu T* (2020) “Distinct utilization of micronutrient biotin in and between adipose and brain during aging is associated with lipogenic shift in Wistar rat brain” Nutr Res June 6. doi: 10.1016/j.nutres.2020.06.004.

Ganesan D, Ramaian Santhaseela A, Rajasekaran S, Selvam S, Jayavelu T* (2020). "Astroglial biotin deprivation under endoplasmic reticulum stress uncouples BCAA-mTORC1 role in lipid synthesis to prolong autophagy inhibition in the aging brain”. J Neurochem Feb 7. doi: 10.1111/jnc.14979.

Senthilraja S, Anand RS, Dhasarathan G, Sudarshana R, Tamilselvan J* (2019). Autophagy inhibition by biotin elicits endoplasmic reticulum stress to differentially regulate adipocyte lipid and protein synthesis. Cell stress chaperones 24: 343-350.

Dasagrandhi D, R ASK, Muthuswamy A, Lennox AM, Jayavelu T, Devanathan V, Kesavan Swaminathan J (2018). Ischemia/reperfusion injury in male guinea pigs: An efficient model to investigate myocardial damage in cardiovascular complications. Biomed Pharmacother 99: 469-479.

Periyasamy S, Sathya M, Karthick C, Kandasamy M, Shanmugaapriya S, Tamilselvan J, Jayachandran KS, Anusuyadevi M (2017). Association Studies of Specific Cholesterol Related Genes (APOE, LPL, and CETP) with Lipid Profile and Memory Function: A Correlative Study Among Rural and Tribal Population of Dharmapuri District, J Alzheimers Dis 60, S195-S207

Rasbach KA, Funk JA, Jayavelu T, Green PT, Schnellmann RG (2010). 5-hydroxytryptamine receptor stimulation of mitochondrial biogenesis. J Pharmacol Exp Ther 332 (2), 632-639.

Tamilselvan J, Jayaraman G, Sivarajan K, Panneerselvam C (2007). Age dependent upregulation of p53 and cytochrome c release and susceptibility to apoptosis in skeletal muscle fiber of aged rats: Role of carnitine and lipoic acid. Free Radic Biol Med 43(12), 1656-1669.

Tamilselvan J, Sivarajan K, Anusuyadevi M, Panneerselvam C (2007) Cytochrome c Oxidase Rather than Cytochrome c is a Major Determinant of Mitochondrial Respiratory Capacity in Skeletal Muscle of Aged Rats: Role of Carnitine and Lipoic Acid. Rejuvenation Res 10(3), 311-326.

Savitha S, Tamilselvan J, Anusuyadevi M, Panneerselvam C (2005) Oxidative stress on mitochondrial antioxidant defense system in the aging process: role of DL-α-lipoic acid and L-carnitine. Clin Chim Acta 355: 173-180.

Samuel S, Kathirvel R, Jayavelu T, Chinnakkannu P (2005) Protein oxidative damage in arsenic induced rat brain: influence of DL-α-lipoic acid. Toxicol Lett 155: 27-34.

Awards/Honors/Positions

Graduate Aptitude Test in Engineering (GATE 2002) qualified
National Eligibility Test (NET 2004) qualified
CSIR – Senior research fellowship award (2007)
Best poster award by Society for Free Radical Research (SFRR)-India (2007)
Biosafety officer” to monitor and recommend recombinant DNA research activities in Anna University
Member of question paper setting committee for all India entrance examination “Biotechnology Eligibility Test (BET 2019)” conducted by Department of Biotechnology, Government of India
Member of question paper setting committee for all India entrance examination “Biotech Industrial Training Programme (BITP 2019-20)” conducted by Department of Biotechnology, Government of India